Category: Research

Matt D Nelson et al. recently authored “An LCD 3D-printed microphysiological system with deflectable extracellular matrix thin films” that was accepted for publication in Biomedical Materials. The article can be found at https://doi.org/10.1088/1748-605X/ae8e15.

Abstract: Microphysiological systems (MPS) have emerged as promising models to better recapitulate in vivo microenvironments, but they often rely on manufacturing techniques that limit their scalability and constrain non-trivial three-dimensional architectures. Here, LCD 3D printing was used to fabricate an MPS capable of microfluidic flow, physiologically relevant cyclic strain, and resistance to rhodamine absorption. The device contains a thin, permeable (readily up to ~70 kDa) extracellular matrix (ECM) substrate ( ~5 µm dehydrated) that can sustain NCI-H441 lung epithelial cells for a period of at least one week. This microfabrication strategy may be extended to different organ systems, enabling researchers to fabricate MPS with more complex designs.

Mark Shad et al. published “Effect of Key Operating Parameters on the Retention and Separation of Ions Using Cyclical Electrical Field Flow Fractionation” in Analytical Chemistry. The full article can be found at https://doi.org/10.1021/acs.analchem.5c03277.

Abstract: The analysis of inorganic ions is critical in the environmental, industrial, and medical fields, with ion chromatography (IC) being the most used method. While IC offers high accuracy and reproducibility, its high cost, complexity, and operational constraints necessitate the development of alternative methods. This study explores the application of CyElFFF, a variant of electrical field-flow fractionation, for ion retention and separation. The CyElFFF with channel dimensions of 25 μm height, 10 cm length, and 0.5 cm width was developed, incorporating a conductivity detector and CO₂ suppressor. Parameters affecting retention time and separation resolution, including injection volume, ion concentration, and electric field conditions, were evaluated. Results showed enhanced ion retention and separation resolution at lower ion concentrations and optimized electric field settings in mode III. Separation of K⁺, Na⁺, NO₃^–, and SO₄^2– was demonstrated with separation resolution improved by reducing frequency and flow rate. These findings establish CyElFFF as a promising approach for ion analysis.

 

Munawar Jawad et al. recently published “A disposable, passive microfluidic cartridge for point-of-care detection of antibodies in total capillary blood based on hemagglutination and machine-learning assisted interpretation” in RSC Advances. 

Abstract: Point-of-care (PoC) detection of antibodies in blood enables rapid, on-site diagnosis. However, these devices often face challenges related to user variability due to the requirement of multiple manual operations. To address this issue, we designed and developed a disposable microfluidic device that requires minimal user input for rapid detection of SARS-CoV-2 antibodies (ABs) in total blood and antigens associated with blood types. Here, we present a passive pressure-driven pumping technique that rapidly mixes blood samples with reagents, delivering results within three minutes. The device requires 15 mL of capillary blood and can detect SARS-CoV-2 ABs across a concentration range of 0 to 60 mg mL−1. Additionally, we demonstrated the versatility of the microfluidic device by implementing blood typing functionality, highlighting its potential for broader serological testing applications. We also developed a support vector machine (SVM) algorithm as a proof-of-concept to demonstrate the potential application of machine learning (ML)-based analysis to complement visual interpretation of results. We evaluated the performance and predictive accuracy of the SVM model and compared it to human interpretations. The analysis showed that the SVM model achieved a statistically significant improvement in predicting varying degrees of agglutination when compared to human interpretation. This device addresses the need for a user-friendly, rapid COVID-19 AB testing solution and blood-typing assay and also provides a model for the future development of diagnostic devices that are integrated with ML models for improved diagnostic accuracy and accessibility in both clinical and non-clinical environments.

A figure of the hemagglutination assay is displayed and the full article can be found at https://doi.org/10.1039/D5RA05719A.

 

Brady Goenner et al. recently published “An open source platform to automate the design, verification, and manufacture of 3D printed microfluidic devices” in Scientific Reports.

Several barriers to widespread microfluidic adoption exist, including high initial fabrication costs and the labor-intensive development process. To address these barriers, a toolchain for the design, verification, and manufacturing of 3D printed microfluidic devices is presented. This work builds on existing electronic design automation (EDA) tools, yielding a toolchain that automatically lays out a microfluidic device from a library of components, simulates the device, and produces a 3D CAD file for manufacture via 3D printing. The process is validated by automatically designing and fabricating a calcium quantification assay. The full article can be found at https://doi.org/10.1038/s41598-025-15976-9.