Authored by Yunhao Peng, Bruce K. Gale, and Himanshu J. Sant, “Waterborne protozoan parasite detection using two-frequency impedance flow cytometry” was recently published in Analytical Methods.
A common cause of gastrointestinal diseases, waterborne parasitic protozoa are micron-sized parasites present in water sources. Therefore, the article outlines the development of a microfluidic water monitoring system based on impedance flow cytometry for the detection of these parasites. By utilizing parallel rather than coplanar electrodes, a limit detection of <0.1% volume ratio is achieved. Additionally, to improve sample discrimination, both a low and high frequency are applied simultaneously, making the method outlined in the article distinct from other proposed systems. A schematic of the monitoring system is displayed in the figure and the full journal article can be found at https://doi.org/10.1039/D5AY00184F.